Heme Coordination Versatility in a Truncated Hemoglobin

Heme, Myoglobin, Hemoglobin

Isoniazid Inhibits the Heme-Based Reactivity of Mycobacterium tuberculosis Truncated Hemoglobin N

Isoniazid represents a first-line anti-tuberculosis medication in prevention and treatment. This prodrug is activated by a mycobacterial catalase-peroxidase enzyme called KatG in Mycobacterium tuberculosis), thereby inhibiting the synthesis of mycolic acid, required for the mycobacterial cell wall. Moreover, isoniazid activation by KatG produces some radical species (e.g., nitrogen monoxide), t...

Modeling truncated hemoglobin vibrational dynamics.

We present a study on the near equilibrium dynamics of two small proteins in the family of truncated hemoglobins, developed under the framework of a Gaussian network approach. Effective beta carbon atoms are taken into account besides Calphas for all residues but glycines in the coarse-graining procedure, without leading to an increase in the degrees of freedom (beta Gaussian Model). Normalized...

Unique Heme-Iron Coordination by the Hemoglobin Receptor IsdB of Staphylococcus aureus

Iron is an essential requirement for life for nearly all organisms. The human pathogen Staphylococcus aureus is able to acquire iron from the heme cofactor of hemoglobin (Hb) released from lysed erythrocytes. IsdB, the predominant Hb receptor of S. aureus, is a cell wall-anchored protein that is composed of two NEAT domains. The N-terminal NEAT domain (IsdB-N1) binds Hb, and the C-terminal NEAT...

The Heme-globin Linkage of Hemoglobin

It was recently reported that CO hemoglobin is relatively more resistant than oxyhemoglobin to digestion by the pancreatic enzymes (l), and that a heme-protein fragment can be precipitated either from the digest solution or from the dialyzed digest by acidification with acetic acid (2). The composition of this CO product has now been investigated. Iron Content-The value of the iron to nitrogen ...